Congratulations to the ProCan® team on their latest publication. This research delves into the optimisation of proteomics and phosphoproteomic biomarker studies using formalin-fixed paraffin-embedded (FFPE) tissues. The team compared four methods to remove the wax and rehydrate these samples and evaluated five extraction methods to identify the most effective approaches for FFPE tissue analysis by proteomics.

They revealed that the Heat n’ Beat method (developed in ProCan®) is the fastest and most reproducible for general protein analysis, while the S-Trap method delivers the highest peptide yield. For phosphopeptide enrichment, TFE stands out as the most efficient.

The study highlights ongoing challenges in quantifying FFPE-derived peptides, such as biases in UV and fluorescence assays, and offers tailored recommendations for method selection based on specific research needs.

By providing a clear set of optimised methods, this research significantly advances the accuracy and reproducibility of biomarker studies.

It brings us closer to unlocking the full potential of FFPE tissues in proteomics for cancer, paving the way for their future clinical application in diagnostics and treatment strategies.

You can read the full publication here: https://lnkd.in/gkvebQrQ